Figure 4: Western blot analysis of the 14-3-3 protein, 22 kDa glycoprotein, GAPDH and prohibitin.
From: Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
Equivalent amounts (20 μg) of proteins from secreted proteins and intracellular proteins were loaded. The changing tendencies of these proteins in different secreted samples were consistent with the iTRAQ analysis. Furthermore, the results of Western blotting showed that fungal cell secreted proteins into the environment in varying degrees. 14-3-3 protein was just observed in secreted samples. Meanwhile prohibitin was only appeared inside cells. The accumulations of 22 kDa glycoprotein and GAPDH showed great difference between intra- and extra- cellular spaces.
