Table 1 List of up-regulated secreted proteins upon hypovirus infection by HPLC-ESI-OrbiTrap MSMS.

From: Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus

No.

Protein ID a

Protein Name

Average change (EP713/EP155) b

Standard error

Unique PepCount c

Cover Percent d

MW e

pI f

 

Ribosomal proteins

491

54955

Small nuclear ribonucleoprotein F

3.3 (−)

0.12

1

9.89%

10327.75

5.3

131

75598

60S ribosomal protein L12

2.2 (−)

0.09

5

34.34%

17645.42

9.41

109

79283

40S ribosomal protein S5

1.8 (↑)

0.07

6

28.84%

23791.27

9.28

154

100785

40S ribosomal protein S14 (CRP2)

1.8 (↑)

0.05

5

39.74%

16045.36

10.68

398

90732

40S ribosomal protein S19 (S16)

1.8 (↑)

0.02

2

13.16%

16681.79

9.18

128

101628

40S ribosomal protein S0 (Ribosome-associated protein 1)

1.7 (↑)

0.05

5

19.30%

31041.9

4.76

173

103417

60S ribosomal protein L30

1.7 (↓)

0.13

4

29.09%

11647.55

9.77

254

103140

60S acidic ribosomal protein P0

1.6 (↑)

0.04

3

9.55%

33639.19

4.62

350

54024

40S ribosomal protein S28

1.6 (↑)

0.1

2

31.34%

7608.81

10.37

 

Oxidoreductase activity

61

93945

Indoleamine 2,3-dioxygenase family protein

2.8 (↓)

0.08

9

16.60%

55419.63

5.43

482

55348

NADPH-dependent D-xylose reductase II, III (XR)

1.8 (−)

0.1

1

3.07%

36430.6

6.15

41

99052

Protein disulfide-isomerase precursor (PDI)

1.7 (−)

0.05

11

26.88%

55317.38

4.69

171

91352

Quinone oxidoreductase

1.7 (−)

0.03

4

19.51%

21569.41

5.46

22

99412

Monodehydroascorbate reductase

1.6 (↓)

0.06

13

29.49%

57358.2

6.38

46

87808

Formate dehydrogenase (NAD-dependent formate dehydrogenase) (FDH)

1.6 (−)

0.02

10

21.56%

48658.25

8.65

118

80754

Superoxide dismutase-1(SOD-1)[Cu-Zn]

1.6 (↓)

0.01

5

37.42%

15945.45

5.85

191

87102

Uricase

1.5 (−)

0.07

4

15.49%

33212.42

6.01

226

107257

NADH-cytochrome b5 reductase

1.5 (↓)

0.09

4

13.49%

37374.71

9

 

Transferase activity

32

42588

UTP-glucose-1-phosphate uridylyltransferase

1.6 (↓)

0.04

12

20.70%

57401.52

6.19

364

58903

4-aminobutyrate aminotransferase

1.6 (↑)

0.04

2

3.64%

51778.91

5.94

373

85870

Glucosamine-fructose-6-phosphate aminotransferase

1.6 (↓)

0.14

2

3.11%

77900.66

6.19

 

Ion binding

161

82952

Pyruvate carboxylase (Pyruvic carboxylase) (PCB)

1.5 (↓)

0.09

5

4.14%

130792.42

6.15

238

70319

Calmodulin (CaM)

1.5 (↑)

0.04

3

11.01%

23424.9

4.45

 

Translation initiation and elongation factors

236

102227

Elongation factor 1-beta

2.7 (↑)

0.11

3

14.85%

25127.97

4.4

24

90536

Elongation factor 2 (EF-2)

1.7 (−)

0.05

13

14.03%

93491.05

6.43

340

93992

Eukaryotic translation initiation factor 3 subunit F (eIF3f)

1.5 (↑)

0.08

2

6.68%

39992.56

4.79

 

ATP synthase

99

82931

ATP synthase subunit alpha, mitochondrial precursor

1.7 (−)

0.02

6

13.04%

59510.29

9.21

152

88657

ATP synthase subunit 5, mitochondrial precursor (Oligomycin sensitivity conferral protein) (OSCP) (ATP synthase chain 5)

1.5 (−)

0.12

5

19.03%

23687.24

9.25

 

Nascent polypeptide-associated complex

167

81890

Nascent polypeptide-associated complex subunit alpha (NAC-alpha) (Alpha-NAC)

2.3 (↑)

0.04

4

22.33%

22291.55

4.72

346

102329

Nascent polypeptide-associated complex subunit beta (NAC-beta) (Beta-NAC)

1.5 (↑)

0.04

2

14.74%

16945.14

5.81

 

Hydrolase activity

376

86663

Phosphatidyl inositol-specific phospholipase C, cplc1 (delta-type PLC)

2.2 (−)

0.03

2

2.31%

79759.42

6.22

303

103361

Aspergillopepsin

1.6 (↓)

0.07

2

8.08%

30918.72

3.75

302

108972

Carboxypeptidase S1

1.5 (↑)

0.11

2

3.72%

52979.56

4.09

 

Others

267

104299

Ubiquitin-conjugating enzyme E2 variant 1

1.7 (↑)

0.24

3

21.43%

17129.33

5.87

54

89402

Protein kinase C(CPKC)

1.5 (−)

0.03

10

11.92%

98880.94

5.24

338

103579

Guanine nucleotide-binding protein subunit beta-like protein (Cross-pathway control WD-repeat protein cpc-2)

1.5 (↑)

0.04

2

7.26%

35040.34

6.55

 

Unknown

337

81491

uncharacterized protein

3.0 (−)

0.06

2

2.03%

110226.61

4.95

188

105888

uncharacterized protein

2.5 (↑)

0.03

4

14.29%

31420.14

4.17

169

96317

uncharacterized protein

2.2 (↓)

0.06

4

24.51%

22018.9

5.58

423

61750

uncharacterized protein

2.2 (↓)

0.08

2

7.35%

29515.04

6.01

163

93265

uncharacterized protein

2.0 (−)

0.03

4

13.42%

31364.56

4.19

126

93693

uncharacterized protein

1.8 (↑)

0.03

5

20.90%

25408.27

5.4

160

90079

uncharacterized protein

1.8 (↓)

0.06

5

14.90%

28492.17

4.9

272

76370

uncharacterized protein

1.7 (−)

0.03

3

8.87%

31368.02

7.2

162

74162

uncharacterized protein

1.6 (↑)

0.03

5

12.85%

46952.71

5.87

477

102698

uncharacterized protein

1.6 (−)

0.07

1

14.46%

9204.46

6.17

110

40356

uncharacterized protein

1.5 (↓)

0.1

6

31.88%

24510.37

4.83

198

84988

uncharacterized protein

1.5 (↓)

0.17

4

17.41%

24885.25

5.05

  1. aAccession number from Cryphonectria parasitica database v1.0.
  2. bHypovirus-free strain EP155 was set as reference. The average change (EP713/EP155) were from three independent experimental data. ‘(−)’, ‘(↑)’, ‘(↓)’ meant mRNA expression level of the corresponding gene was unchanged, up-regulated, down-regulated respectively upon hypovirus infection using high-throughput sequencing by Genome Analyzer IIx (Illumina).
  3. cThe total number of unique matched peptides to the identified protein.
  4. dThe cover percentage of matched peptides to the protein sequence.
  5. eTheoretical molecular weight.
  6. fTheoretical pI.
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