Table 2 List of down-regulated secreted proteins upon hypovirus infection identified by HPLC-ESI-OrbiTrap MSMS.

From: Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus

No.

Protein ID a

Protein Name

Average change (EP155/EP713) b

Standard error

Unique PepCount c

Cover Percent d

MW e

pI f

 

Hydrolase activity

172

36288

Endopolygalacturonase 1 precursor (Pectinase) (Clpg1)

3.7 (↑)

0.23

4

9.46%

37662.76

6.58

331

73874

Secreted aspartic proteinase

3.6 (↑)

0.3

2

3.55%

59382.36

4.07

487

95002

Endoglucanase-4 precursor (Endoglucanase IV) (Endo-1,4-beta-glucanase IV) (Cellulase IV) (EGIV)

3.0 (↓)

0.32

1

1.94%

36058.34

4.34

230

108053

Cutinase precursor (Cutin hydrolase)

2.8 (−)

0.42

3

18.42%

29225.54

4.19

492

69709

ATP-dependent DNA helicase II, 70 kDa subunit

2.8 (↓)

0.09

1

2.59%

39932.8

4.88

121

105629

Extracellular chitinase

2.4 (↑)

0.13

5

10.43%

48705.46

4.61

349

43693

Alpha-glucosidase precursor (Maltase)

2.4 (↓)

0.21

2

1.54%

111602.21

4.49

480

79318

3-phytase A precursor (Myo-inositol-hexaphosphate 3-phosphohydrolase A) (3 phytase A) (Myo-inositol hexakisphosphate phosphohydrolase A)

2.3 (↑)

0.42

1

2.08%

52879.6

4.82

315

107379

Beta-fructofuranosidase

2.0 (↓)

0.12

2

2.74%

62995.83

4.58

323

84745

Alpha amylase

2.0 (−)

0.08

2

3.17%

58630.95

4.19

327

99792

Extracellular cell wall glucanase

2.0 (↑)

0.36

2

9.92%

27691.24

4.11

165

67775

GPI-anchored cell wall beta-1,3-endoglucanase EglC

1.9 (↓)

0.07

4

18.16%

40242.32

4.41

233

107093

Endothiapepsin precursor (Aspartate protease)

1.6 (−)

0.13

3

6.63%

56426.06

4.43

305

103771

Survival protein sure-like phosphatase/nucleotidase-like protein

1.6 (↓)

0.08

2

7.79%

31859.39

4.05

304

65827

Polygalacturonase-3 precursor (Polygalacturonase III) (PG-III) (PGC) (Pectinase-3)

1.5 (−)

0.16

2

10.73%

36227.1

3.8

 

Oxidoreductase activity

474

39428

Short chain dehydrogenase/reductase family oxidoreductase

2.9 (↑)

0.2

1

2.75%

31682.42

6.53

26

106275

Alcohol dehydrogenase-1(ADH-1)

2.3 (↑)

0.1

12

38.70%

37278.81

6.15

66

39110

Cellobiose dehydrogenase

1.8 (−)

0.08

8

15.44%

61477.37

5.32

182

97515

Coproporphyrinogen III oxidase

1.8 (↓)

0.17

4

9.90%

46496.86

7.66

241

71516

Aspartate-semialdehyde dehydrogenase

1.7 (−)

0.2

3

7.12%

39149.09

6.2

10

101684

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

1.5 (−)

0.03

18

59.76%

36191.85

6.46

101

64525

NADP-dependent leukotriene B4 12-hydroxydehydrogenase

1.5 (↑)

0.1

6

16.89%

31463.95

6.46

359

83754

Laccase

1.5 (↑)

0.09

2

3.99%

63159.75

4.36

388

104397

Putative oxidoreductase

1.5 (↑)

0.11

2

6.67%

39540.91

5.83

 

Transferase activity

345

48583

Serine hydroxymethyltransferase, cytosolic (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT)

1.8 (−)

0.22

2

4.48%

51419.79

6.95

243

108828

1,3-beta-glucanosyltransferase gel2 precursor (Glucan elongating glucanosyltransferase 2)

1.9 (↓)

0.7

3

7.79%

48862

4

325

41127

Ribokinase-like protein

1.5 (↑)

0.17

2

6.80%

36364.05

5.1

 

Lypase/Esterase activity

119

105554

Extracellular lipase

2.5 (↑)

0.2

5

19.08%

37288.15

4.17

202

95432

Arylsulfatase

2.3 (−)

0.07

4

5.56%

69618.99

4.82

279

97118

Carboxylesterase

3.6 (↑)

0.19

3

8.23%

60638.58

4.36

 

Isomerase activity

56

55987

Peptidyl-prolyl cis-trans isomerase, mitochondrial precursor (PPIase) (Rotamase) (Cyclophilin) (Cyclosporin A-binding protein) (CPH)

2.1 (−)

0.07

9

43.41%

20099.5

7.81

244

58361

Peptidyl-prolyl cis-trans isomerase B precursor (PPIase B) (Rotamase B)

1.7 (−)

0.29

3

17.92%

18877.67

6.98

 

Lyase activity

75

104720

fructose bisphosphate aldolase

1.8 (↓)

0.24

7

22.71%

39353.73

5.52

 

Regulation factors

133

93073

mRNA binding post-transcriptional regulator

1.7 (−)

0.18

5

14.66%

41167.13

8.6

151

102966

14-3-3 protein

1.6 (↑)

0.02

5

20.51%

30396.05

4.9

142

40411

RHO protein GDP dissociation inhibitor(RDI) regulation of growth rate

1.5 (↑)

0.01

5

26.87%

22567.15

5.49

 

Ribosomal proteins

261

86511

Ribosomal protein L28e

2.4 (↑)

0.26

3

16.45%

16509.76

11

55

103787

Ubiquitin-40S ribosomal protein

1.8 (−)

0.02

9

45.81%

17687.54

9.85

 

Cytoskeleton

263

76520

Microtubule-associated protein RP/EB family member 1

1.9 (↑)

0.37

3

12.70%

27527.94

4.76

311

102150

Profilin

1.7 (−)

0.24

2

15.91%

13559.13

5.73

 

Unknown

322

107212

uncharacterized protein

2.9 (↓)

0.36

2

9.18%

29454.98

5.74

313

67753

uncharacterized protein

2.6 (↓)

0.16

2

8.33%

25705.21

4.28

232

74613

uncharacterized protein

2.5 (↓)

0.17

3

20.33%

18468.21

4.43

234

103448

uncharacterized protein

2.3 (↓)

0.16

3

13.83%

40306.61

4.8

100

95286

uncharacterized protein

1.9 (↓)

0.14

6

11.96%

51317.18

9.71

308

74957

uncharacterized protein

1.9 (−)

0.35

2

9.14%

36457.53

4.08

301

97441

uncharacterized protein

1.8 (−)

0.02

2

8.14%

35189.2

3.77

148

82357

uncharacterized protein

1.7 (↑)

0.25

5

17.65%

23990.76

6.65

312

104348

uncharacterized protein

1.7 (↓)

0.2

2

3.49%

74787.31

4.62

199

107223

uncharacterized protein

1.6 (↑)

0.22

4

6.58%

73713.63

4.49

207

70844

uncharacterized protein

1.5 (−)

0.23

4

20.20%

20542.29

4.92

  1. aAccession number from Cryphonectria parasitica database v1.0.
  2. bHypovirus-free strain EP155 was set as reference. The average change (EP155/EP713) were from three independent experimental data. ‘(−)’, ‘(↑)’ and ‘(↓)’ mean mRNA expression level of the corresponding gene was unchanged, up-regulated, down-regulated respectively upon hypovirus infection using high-throughput sequencing by Genome Analyzer IIx (Illumina).
  3. cThe total number of unique matched peptides to the identified protein.
  4. dThe cover percentage of matched peptides to the protein sequence.
  5. eTheoretical molecular weight.
  6. fTheoretical pI.
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