Figure 3
From: Sequestration of ubiquitous dietary derived pigments enables mitochondrial light sensing
Light and PA regulates lipid composition in C. elegans.
(A) Triglyceride quantification in light exposed animals in the presence or absence of PA at 2 days of age. Replicate measures are shown with averages and 95% confidence intervals. *P-value < 0.05 determined by unpaired, two sided, Student’s t-test. (B) Total free fatty acid quantification in worms described above. (C) UPLC profiles illustrating the relative amounts of fatty acids in light exposed animals in the presence or absence of PA at 2, 4 and 6 days of age. Free fatty acids represent the free fatty acids in the worm, while the total fatty acid profile represents free fatty acids obtained after saponification of a worm extract. Each peak represents a different free fatty acids. Peaks eluting later in time represent longer fatty acid chain lengths or more hydrophobic lipids. Numbers above the peaks represent the percent change in peak area (i.e. fatty acid amount) between the light exposed PA treated and PA naïve animals. Arrows point to peaks with the same area, to which curves where normalized.
