Figure 6: Neurons derived from P19 cells treated with EX-527 have electrophysiological activities. | Scientific Reports

Figure 6: Neurons derived from P19 cells treated with EX-527 have electrophysiological activities.

From: A potent and selective small molecule inhibitor of sirtuin 1 promotes differentiation of pluripotent P19 cells into functional neurons

Figure 6

(a) P19 cells were incubated with 100 μM EX-527 for 14 days. Whole-cell currents in the differentiated cells were measured by depolarizing voltage ranged from −60 mV to +20 mV, with a 10-mV step before (left) and after (right) 0.5 μM TTX perfusion (n = 6/10 of recorded cells). The scales of time and current are presented. Insets show respective traces on an expanded scale. (b) P19 cells were incubated with 100 μM EX-527 for 14 days. Action potentials in the differentiated cells were recorded in response to depolarization by current injection before (left) and after (right) 0.5 μM TTX perfusion (n = 6/10 of recorded cells). The scales of time and voltage are presented.

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