Figure 2
NLRP3 inflammasome activation in heart tissue.
(A) MDA level in cardiac tissue. (B) MnSOD activity in cardiac tissue. (C) Western blot images of 4-HNE and MnSOD. (D) Quantitative band density analyses of 4-HNE and MnSOD. (E) Representative images of cardiac sections stained with NLRP3 (green) together with DNA staining with DAPI (blue), scale bar = 200 μm. (F) Representative images of cardiac sections stained with Il-1β (red) together with DNA staining with DAPI (blue), scale bar = 200 μm. (G) Representative Western blot images for NLRP3, ASC-1, Pro-caspase 1, Cleaved-caspase-1, Pro-IL-1β and Mature-IL-1β. (H) Quantitative band density analyses of NLRP3, ASC-1, caspase-1 and IL-1β. The results are presented as the fold increase in the ND group. The data are means ± SEM (n = 12). *p < 0.05 vs. ND, **p < 0.01 vs. ND; #p < 0.05 vs. HFD, ##p < 0.01 vs. HFD. MDA: Malondialdehyde; Mn-SOD: Mn-superoxide dismutase.
