Figure 3
From: Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress
VP-16 reduces collagen deposition in LX-2 cells.
(a–c) LX-2 cells were treated with various concentrations of VP-16 for 72 h. (a) The mRNA expression levels of type I collagen and α-SMA were determined using real-time PCR. (b) The protein levels of type I collagen and α-SMA were measured by western blotting. β-actin was used as a loading control. (c) VP-16 regulates the expressions of TIMP-1 and MMP-13, as shown by western blotting analysis. β-actin was used as a loading control. The data are presented as the mean ± SD of three independent experiments performed in triplicates. *P < 0.05 ***P < 0.001.
