Figure 5
From: Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress
VP-16 had a more potent effect on LX-2 cells than normal hepatocytes.
(a–c) Three cell lines were treated with 0–4 μM VP-16 for 72 h. (a) Cytotoxicity was measured using the CCK-8 assay. (b) Apoptotic cells were analyzed using flow cytometry. Annexin V-positive cells are displayed in the bar chart as a percentage of the untreated controls. (c) Apoptosis-regulating protein levels were analyzed by western blotting. β-actin was used as a loading control. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, ***P < 0.001.
