Figure 1
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Luciferase reporter activity assay identifies let-7f-5p as a regulator of MYH9.
(a) Pig MYH9 3′UTR and monkey MYH9 3′UTR were cloned into 3′UTR-luciferase reporter psiCheck2 vectors to create plasmids designated psiCheck2-pMYH9-WT and psiCheck2-mMYH9-WT, respectively. (b) HEK293FT cells were cotransfected with indicated mimics and psiCheck2-pMYH9-WT. (c) HEK293FT cells were cotransfected with indicated mimics and psiCheck2-mMYH9-WT. Thirty-six hrs later, cells were lysed and luciferase activity was measured. Results are expressed as mean ± SD of three independent experiments. p values were calculated using the Student’s t test. **P < 0.01, ***P < 0.001. (d) Sequence homology of let-7f-5p to human, monkey and pig.
