Figure 2

Let-7f-5p specifically binds to the 3′ UTR of pig MYH9.

(a) The 3′UTR of pig MYH9 contains one seed-matched target site (highlighted) for let-7f-5p. (b) Schematic of the “seed region” match between let-7f-5p and pig MYH9 3′UTR. The mutation of six nucleotides in the seed match is shown. The positions of six seed match sites for let-7f-5p in the pig MYH9 3′UTR were replaced. (c) WT or MUT reporter plasmids were cotransfected with the let-7f-5p mimics, let-7f-5p-MUT mimics, or NC mimics into the HEK293FT cells. Reporter activities were determined thirty-six hrs post-transfection by dual-luciferase assays. Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. ***P < 0.001. (d) Sequence homology of the let-7f-5p binding sequence in the MYH9 3′UTR of human, monkey and pig.