Figure 5
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Overexpression of let-7f-5p decreases MYH9 mRNA and protein levels in PAMs.
PAMs were transfected with 100nM of MYH9-siRNA, NC-siRNA, let-7f-5p mimics and NC-mimics. Relative expression level of let-7f-5p (a) and MYH9 mRNA (b) in PAMs determined by qRT-PCR. MYH9 protein expression (c) analyzed by western blot with α-tubulin as the control. Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. **P < 0.01, ***P < 0.001.
