Figure 6
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Inhibition of let-7f-5p enhances MYH9 mRNA and protein expression in PAMs.
PAMs were transfected with indicated concentrations of MYH9-siRNA, NC-siRNA, let-7f-5p-inhibitor and NC-inhibitor. Relative expression level of let-7f-5p (a) and MYH9 mRNA (b) in PAMs determined by qRT-PCR. MYH9 protein expression (c) analyzed by western blot, with α-tubulin as control. Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. *P < 0.05, **P < 0.01.
