Figure 7
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Let-7f-5p is reduced after PRRSV infection.
(a) Heatmap for let-7 family members at 12 hpi compared with control group in the PAMs infected with two PRRSV strains, GD-HD and CH-1a (MOI = 0.1), quantified by microRNA deep sequencing. The log2 fold changes were used to plot the heatmap. PAMs cells were mock-infected or were infected with UV-inactivated or active forms of two PRRSV strains, GD-HD and CH-1a (MOI = 0.1). PRRSV ORF7 (b,c), let-7f-5p (d,e) and MYH9 (f,g) mRNA relative expression levels were determined using qRT-PCR. Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.
