Figure 9
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Overexpression of let-7f-5p attenuates both HP-PRRSV and N-PRRSV replication in PAMs.
PAMs were transfected with 100 nM let-7f-5p or NC mimics, followed by infection with two PRRSV strains: GD-HD and CH-1a (MOI = 0.01). (a,b) Relative expression level of MYH9 mRNA was evaluated by qRT-PCR. PRRSV replication was evaluated by qRT-PCR for intracellular PRRSV ORF7 RNA (c,d) and extracellular PRRSV genome RNA in the supernatants (e,f) and viral titers in the supernatants were measured by TCID50 at the indicated time points (g,h). Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.
