Table 3 Primers used for qRT-PCR and psiCheck2 vector construction.
From: MicroRNA let-7f-5p Inhibits Porcine Reproductive and Respiratory Syndrome Virus by Targeting MYH9
Primer name | Sequence |
|---|---|
let-7f-RT-Primer | 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACTATA-3′ |
let-7f-Stem-Loop-F | 5′-ACACTCCAGCTGGGTGAGGTAGTAGATTGTA-3′ |
let-7f-Stem-Loop-R | 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAG-3′ |
U6-RT-Primer | 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATATGG-3′ |
U6-Stem-Loop-F | 5′-CTCGCTTCGGCAGCACA-3′ |
U6-Stem-Loop-R | 5′-AACGCTTCACGAATTTGCGT-3′ |
pMYH9-qF | 5′-AAGGCACCGTCAAGTCCAA-3′ |
pMYH9-qR | 5′-TTCCTCCGCTCATCATCCA-3′ |
PRRSV-ORF7-gene-F | 5′-AGATCATCGCCCAACAAAAC-3′ |
PRRSV-ORF7-gene-R | 5′-GACACAATTGCCGCTCACTA-3′ |
HPRT1-F | 5′-TGGAAAGAATGTCTTGATTGTTGAAG-3′ |
HPRT1-R | 5′-ATCTTTGGATTATGCTGCTTGACC-3′ |
psiCheck2-mMYH9-3UTR-F | 5′-TTACTCGAGGCCTCTTCTCCTGCAGCCT-3′ |
psiCheck2-mMYH9-3UTR-R | 5′-TTGCGGCCGCTTTTTGGTAGCACATGGTTCTCTT-3′ |
psiCheck2-pMYH9-3UTR-F | 5′-TTACTCGAGCAGCCTGCTCTTCTCCTGCT-3′ |
psiCheck2-pMYH9-3UTR-R | 5′-TTGCGGCCGCTGTTGGGAGAAGCCACGGCT-3′ |
psiCheck2-mMYH9-3UTR-Mut-F | 5′-TCGAGTGCTGTTGGCAATCACACGTGGTGTGGAGTCACACCTCTGCCCCTTGGGCCGC-3′ |
psiCheck2-mMYH9-3UTR-Mut-R | 5′-GGCCGCGGCCCAAGGGGCAGAGGTGTGACTCCACACCACGTGTGATTGCCAACAGCAC-3′ |
psiCheck2-pMYH9-3UTR-Mut-F | 5′-TCGAGTGCTGTTGGCAATCACACGCGGTGTGGAGTCACCCTCTGCCCCCCCCAGCCGC-3′ |
psiCheck2-pMYH9-3UTR-Mut-R | 5′-GGCCGCGGCTGGGGGGGGCAGAGGGTGACTCCACACCGCGTGTGATTGCCAACAGCAC-3′ |
