Figure 4: ITC Reveals Physical Binding of Phospho-YxxP Peptides to Monosiga crka1 SH2 Domain.
From: A pre-metazoan origin of the CRK gene family and co-opted signaling network
Values indicate the binding affinity as a dissociation constant (Kd). Kd values are arbitrarily categorized as high affinity (Kd < 3 μM, red), moderate affinity (Kd between 3–20 μM, yellow), or low affinity (Kd > 20 μM, blue). Kd values are not shown in cells shaded gray due to poor curve fitting (PCF). See titration plots in Supplementary Figure S9. ND, not determined.
