Figure 3 | Scientific Reports

Figure 3

From: Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan

Figure 3

Overnight hydrolysis (14 hours) of xylan substrates (WAX, RAX and OSX) by the five putative endoxylanases.

The experiments were carried out by incubating 5 mg/ml xylan substrate with each endoxylanase at final concentration of 0.5 μM at 37 °C for 14 hours, the products of hydrolysis were identified and quantified by HPLC, Arabinose (A1), xylose (X1) and xylo-oligosaccharides (X2 to X6) were mixed and analyzed by HPLC to serve as standards for the assignment of the released products. Calibration curves were constructed with known concentrations of A1 and X1-X6. The concentrations of each sugar were calculated by fitting the peak area into calibration curve.

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