Figure 4 | Scientific Reports

Figure 4

From: Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan

Figure 4

Functional analyses of B. intestinalis BiXyl43A and BiAgu67A.

(A) Hydrolysis of xylo-oligosaccharides (X2–X6) by BiXyl43A. Purified BiXyl43A was incubated with different xylo-oligosaccharides and the hydrolytic products were analyzed by HPLC. (B) Hydrolysis of aldouronic acids by BiXyl43A and BiAgu67A. The activity of the BiAgu67A was determined by the function to release xylo-oligosaccharides from aldouronic acids and its capacity to release xylose from aldouronic acids when act synergistically together with a β-xylosidase (BiXyl43A). The reaction products were analyzed by HPLC and identified by compared with peaks with retention times of xylo-oligosaccharides as standards.

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