Figure 5 | Scientific Reports

Figure 5

From: Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan

Figure 5

Overnight synergistic hydrolysis (14 hours) of xylan substrates of multiple endoxylanases with BiXyl43A and BiAgu67A towards WAX, RAX and OSX.

The synergistic effect of the xylanases with the two putative accessory enzymes (BiXyl43A and BiAgu67A) were investigated by incubating BiXyl43A and BiAgu67A with each endoxylanase together at 37 °C for 14 hours, the synergistic hydrolysis experiments of all seven enzymes were performed as well. The final concentration for each enzyme was 0.5 μM, the final hydrolysis products were identified and quantified by HPLC, Arabinose (A1), xylose (X1) and xylo-oligosaccharides (X2 to X6) were mixed and analyzed by HPLC to serve as standards for the assignment of the released products. Calibration curves were constructed with known concentrations of A1 and X1–X6. The concentrations of each sugar were calculated by fitting the peak area into calibration curve.

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