Figure 5
Comparative phylogenetic analysis of male and female timing parameters.
(A) Unrooted neighbor-joining (NJ) tree of 17 E. diurnus populations from southern France and northeastern Spain (Fig. 4A) generated from microsatellite loci. Scale at bottom left indicates 0.05 nucleotide substitutions per site; values over branches represent posterior probabilities. Red and blue bars to the right of each population indicate the values of m (minimum post-stimulus call delay in males) and f (maximum leader-follower call separation for which females prefer the leader); see Figs 3B,C and 4B. syl is mean syllable number in the male call. (B) Correlation between m and f among the 17 populations corrected by phylogenetically independent contrasts (PIC). Black line is ordinary least-squares linear regression through the origin for the 16 standardized, positivized contrasts (ρ = 0.76, p < 0.001; df reduced by 2 to account for polytomy); red line is reduced major axis regression. (C) Unrooted NJ tree of 7 genetically distinct clusters determined via Bayesian clustering (Fig. 6). Each cluster comprises 1–4 of the 17 populations. (D) Correlation between mean m and f among the 7 clusters from Fig. 5C corrected by PIC (ρ = 0.94, p < 0.01; n = 6 contrasts).
