Figure 3 | Scientific Reports

Figure 3

From: Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro

Figure 3

Intracellular ALDH3A1 levels, analyzed using imaging flow cytometry.

(a) Box and whisker plot of ALDH3A1 fluorescence intensity (see also legend to Fig. 2). The median fluorescence intensity can be considered as a relative measure of intracellular ALDH3A1 levels. *p < 0.001 compared to CON+; ^p < 0.001 compared to CON− (Mann-Whitney rank sum tests). (b) Representative images of keratocytes labeled for ALDH3A1, as revealed using imaging flow cytometry (orange, AlexaFluor® 488, ALDH3A1). For details of conditions, see legend to Fig. 1.

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