Figure 5 | Scientific Reports

Figure 5

From: Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro

Figure 5

Representative microscopy images of human corneal stromal cells activated by TGF-β2 (corneal stromal wound model) and under treatments as defined in the legend to Fig. 1.

(a) Laser scanning confocal microscopy of transdifferentiation of keratocytes to myofibroblasts after exposure to 10 ng/ml TGF-β2, showing labeling of α-SMA (green, AlexaFluor® 488) and nuclei (red, DAPI). (b) Phase-contrast light microscopy of isolated primary human corneal keratocytes and corneal myofibroblasts after exposure to 10 ng/ml TGF-β2. (c,d) Representative laser scanning confocal microscopy images following treatments of keratocytes as detailed in legend to Fig. 1, showing labeling of Thy-1 (blue, PE/Cy5®), α-SMA (green, FITC) and nuclei (red, DAPI). Scale bars, 100 μm. Images are representative of three independent experiments.

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