Figure 5
From: Improved Cryopreservation of Human Umbilical Vein Endothelial Cells: A Systematic Approach

Membrane integrities of HUVECs after graded freezing using a 1 °C/min cooling rate to various sub-zero temperatures in the presence of 10% DMSO, 20% DMSO, 10% DMSO + 5% HES, or 10% DMSO + 8% HES.
Three independent experiments were carried out and the mean membrane integrity was calculated for each experimental temperature. Error bars represent standard error of the mean.