Figure 7
From: Improved Cryopreservation of Human Umbilical Vein Endothelial Cells: A Systematic Approach
(a) Representative phase contrast images of tube formation and (b) membrane integrity and tube lengths normalized against control cells. HUVECs were subjected to graded freezing at 1 °C/min cooling rate in the presence of 5% DMSO and 6% HES, cooled to various sub-zero temperatures, plunged into liquid nitrogen, rapidly thawed and plated on Matrigel. Images were acquired at 40X magnification. The total tube length in the cryopreserved samples was quantified (in pixels) using the NIH ImageJ software with the Angiogenesis Analyzer plugin and normalized against fresh, unfrozen (control) cells. Likewise, the membrane integrity was normalized against unfrozen (control) cells. P-values indicate that normalized membrane integrity and tube-forming ability are not significantly different for all plunge temperatures tested.
