Table 1 Membrane integrity of HUVEC controls in the presence and absence of DMSO.
From: Improved Cryopreservation of Human Umbilical Vein Endothelial Cells: A Systematic Approach
Measured membrane integrity | In the absence of cryoprotectant | 10% DMSO, 15 minute exposure | 10% DMSO, 30 minute exposure | 10% DMSO, multi-step exposure15 | 20% DMSO, 15 minute exposure |
|---|---|---|---|---|---|
Pre-experiment | 92.9 ± 0.7% | 93.3 ± 0.7% | 91.4 ± 1.3% | 93.0 ± 0.5% | 84.6 ± 0.4% |
After 1 hour at 0 °C | 90.6 ± 1.2% | 91.7 ± 1.5% | 90.5 ± 0.9% | 91.0 ± 2.2% | 77.1 ± 2.4% |
Plunge from 0 °C into liquid nitrogen | 0.07 ± 0.03% | 0.8 ± 0.3% | 2.1 ± 0.7% | 4.6 ± 0.3% | 2.0 ± 0.2% |
