Table 2 Maximum membrane integrities of HUVECs after incubation with various cryoprotectants for 15 minutes on ice, cooling to the nucleation temperature, nucleating ice, holding for 3 minutes and then cooling at 1 °C/min to various sub-zero temperatures, plunging into liquid nitrogen and then thawing.
From: Improved Cryopreservation of Human Umbilical Vein Endothelial Cells: A Systematic Approach
Cryoprotectant(s) | Plunge temperature (°C) | Maximum observed membrane integrity (%) | Membrane integrity normalized to fresh control |
|---|---|---|---|
10% DMSO | −35 | 67.4 ± 1.9 | 72.6 ± 1.8 |
20% DMSO | −40 | 59.2 ± 2.2 | 64.3 ± 1.6 |
10% DMSO + 10% HES | −45 | 78.7 ± 0.5 | 87.8 ± 2.0 |
10% DMSO + 8% HES | −45 | 83.6 ± 1.6 | 91.2 ±1.3 |
10% DMSO + 5% HES | −45 | 78.8 ± 0.9 | 83.6 ± 1.0 |
7% DMSO + 7% HES | −45 | 81.2 ± 2.2 | 87.4 ±2.2 |
7% DMSO + 6% HES | −45 | 77.7 ± 0.8 | 83.2 ± 0.5 |
5% DMSO + 6% HES | −35 | 87.7 ± 0.8 | 94.0 ± 0.9 |
3% DMSO + 6% HES | −35 | 79.3 ± 2.1 | 85.2 ± 2.0 |
Commercially-supplied | 64.8 ± 2.2 |
