Figure 1: ClpV1-ClpV2 interchangeability.

(A) Sci-1-dependent antibacterial growth inhibition. Prey cells (W3110 gfp⁺, kan^R) were mixed with the indicated attacker cells, spotted onto sci-1-inducing medium (SIM) agar plates and incubated for 4 hours at 37 °C. (B) Sci-2-dependent antibacterial growth inhibition. Prey cells (W3110 gfp⁺, kan^R) were mixed with the indicated attacker cells, spotted onto Dulbecco’s modified Eagle Medium (DMEM) agar plates and incubated for 4 hours at 37 °C. The image of a representative bacterial spot is shown and the relative fluorescent levels (in arbitrary units, AU) are indicated in the upper graph. The number of recovered E. coli prey cells is indicated in the lower graph (in log₁₀ of colony-forming units (cfu)). The circles indicate values from three independent assays, and the average is indicated by the bar.