Figure 5: Crystal structure of the ClpV1 N-terminal domain complexed to the TssC1 N-terminal helix.

(A,B) Ribbon view of the ClpV1-Nt/TssC1 peptide complex. The ClpV1 N-terminal domain is rainbow colored and the TssC1 peptide is shown in beige. The view in (B) is rotated by 90° compared to (A) and the uncomplexed ClpV1 N-terminal domain is superimposed and colored grey. (C) Surface representation of the complex. The ClpV1 N-terminal domain is rainbow colored and the TssC1 peptide is colored beige. (D) Surface view of the ClpV1 N-terminal domain (green) in complex with the TssC1 peptide represented as a ribbon (beige). The inset shows a close-up of the binding crevice, with the surface colored according to electrostatics (blue/red, positively/negatively charged residues; white, hydrophobic residues). (E) Detailed ball-and-stick scheme of the interaction. The side chains of the residues at the ClpV1-TssC1 interface of the ClpV1 N-terminal domain (blue area) are represented with brown bonds whereas that of the TssC1 peptide (grey area) are represented with violet bonds. TssC1 residues are indicated in blue whereas the side chains of ClpV1 residues involved in the interaction are indicated in green (scheme made with Ligplot+⁶⁵).