Figure 7: The changes of cell differentiation and morphogenesis in random samples of 300 μm&PD+ –printed construct when shift to 2D culture at day 14 of culture. | Scientific Reports

Figure 7: The changes of cell differentiation and morphogenesis in random samples of 300 μm&PD+ –printed construct when shift to 2D culture at day 14 of culture.

From: 3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland

Figure 7

Epidermal progenitor cells embedded in 3D-printed constructs differentiation into sweat gland cells by immunostaining assay at day 5 of culture with K18 and aggregation into sweat gland-like structure at day 14 of culture. All markers are red and Dapi staining of nuclei is blue. After shifting to 2D culture, the structure disappearance at day 21 of culture and differentiated cells decrease at day 28 of culture (scale bar, 50 μm).

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