Figure 5
E-selectin/ligands are required for EPC-dependent blood vessel formation.
(A) Wound blood vessel perfusion with Dil dye. Upper: representative images of Dil-stained wound blood vessels detected by confocal laser scanning photography at day 7 are shown for each type of recipient mice (I–IV as illustrated in Fig. 4A). Lower: Quantification of vessel density in the entire area of residual wounds on day 7, as percent fluorescence. (B) Upper: representative images of non-invasive LDI measurements showing spontaneous restoration of blood flow into ischemic hindlimbs after femoral artery ligation/excision in different recipient mice (I–IV as illustrated in Fig. 4A). Lower: Quantitative data of LDI measurements. Ratio of ischemic versus normal hindlimb between two groups of mice at various time points. For both (A,B), data are analyzed by two-way ANOVA test and presented as mean ± SEM from each group (n = 6/group).
