Figure 2 | Scientific Reports

Figure 2

From: Improving protein content and quality by over-expressing artificially synthetic fusion proteins with high lysine and threonine constituent in rice plants

Figure 2

Molecular characterization of 35S::TKTKK1 transgenic plants.

(a) Copy number detection of T-DNA insertion in transgenic plants by Southern blot hybridization. DNA samples from a total of 18 transgenic plants were restricted by EcoRV and then transferred onto nylon membrane for hybridization using the HPT probe. The red triangle indicated the lines with single copy of T-DNA insertion. (b) Bar diagrams showing expression patterns of the TKTKK1 gene in the 18 independent transgenic plants by qRT-PCR. The mRNA relative amount (Y axis) was calculated according to the description in Materials and methods. The amplification of an eEF-1a gene was used as an internal control to normalize the data. The red arrows in (a) indicated the lines with single copy of T-DNA insertion by Southern blotting analysis. The red stars “*” in (a, b) indicated the lines with single copy of T-DNA insertion and with relatively higher expression level. These three independent lines were selected for further investigation.

Back to article page