Figure 5 | Scientific Reports

Figure 5

From: Improving protein content and quality by over-expressing artificially synthetic fusion proteins with high lysine and threonine constituent in rice plants

Figure 5

Preliminary analysis of lysine and crude protein and FST analysis.

(a,b) Lysine and crude protein content. respectively, in T0 generation of transgenic plants carrying the construct 35S::TKTKK1. (c) Characterization of FSTs of T-DNA insertion. A total of 5 independent lines from 35S::TKTKK1, 35S::TKTKK2 or 35S::TKTKK3 were selected for FST analysis. NA, not available. (d) Schematic diagram for PCR genotyping T1 or T2 generation of transgenic plants. Forward (F) and reverse (R) primers were designed according to the FSTs. Left border reverse (LBR) and right border forward (RBF) primers were designed according to T-DNA border sequence. (e) Identification of three different genotypes including heterozygotes, homozygotes and WT in T1 or T2 transgenic plants based on PCR results. The symbol “+” indicated that PCR fragment could be amplified using corresponding primer sets and “−” indicated no PCR product. (f) Genotyping of five populations from 5 T1 generations of transgenic plants and their χ2 test.

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