Figure 3: Changes in modified redox-sensitive proteins during recovery after H2O2 treatment. | Scientific Reports

Figure 3: Changes in modified redox-sensitive proteins during recovery after H2O2 treatment.

From: Degradation of Redox-Sensitive Proteins including Peroxiredoxins and DJ-1 is Promoted by Oxidation-induced Conformational Changes and Ubiquitination

Figure 3

(a) Representative sliver-stained 2D-gel images on a non-linear pH gradient (4–7) of each redox-sensitive proteins in MDA-MB-231 cells during recovery after treatment (control or 0.5 mM H2O2 in HBSS for 30 min). (b) Each protein spot at reduced and oxidized status was quantified and data from triplicate experiments are presented. (c) Western analysis of Prx2 in (a) sample during recovery time after 0.5 mM H2O2 treatment using anti-Prx2 antibody (left panel) and anti-Prx-SO2/3H antibody (right panel).

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