Figure 4: Post-translational modifications of redox-sensitive proteins in 2D-PAGE spots. | Scientific Reports

Figure 4: Post-translational modifications of redox-sensitive proteins in 2D-PAGE spots.

From: Degradation of Redox-Sensitive Proteins including Peroxiredoxins and DJ-1 is Promoted by Oxidation-induced Conformational Changes and Ubiquitination

Figure 4

(a) 2D-PAGE diagram of spot changes of redox-sensitive proteins after 0.5 mM H2O2 treatment for 30 min. Spot 1 is an original control protein. Spot 2 is a control spot and spot 3 an oxidized one shifted to acidic side, after H2O2 treatment. Spot 4 is an original protein and spot 5 is an oxidized one in 24 h recovery time after H2O2 treatment. (b) Identification of protein modifications in each spot of redox-sensitive proteins in (a) by peptide sequencing with MS/MS.

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