Figure 1: Schematic representation of the different designs of GECIs, composition of the original library, and suggested stages of NTnC Ca2+ indicator function. | Scientific Reports

Figure 1: Schematic representation of the different designs of GECIs, composition of the original library, and suggested stages of NTnC Ca2+ indicator function.

From: A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

Figure 1

(a) Schematic representation of FRET-based, cpFP-based, and NTnC sensor families in the Ca2+-bound state. FPs are shown as cylinders, and tsTnC, CaM and M13-peptide are shown in dark grey, light grey, and speckled grey, respectively. (b) The original library for NTnC consisted of the sensory C-terminal minimal domain of TnC (tsTnC) inserted into the mNeonGreen fluorescent protein between residues 145 and 146, with randomized linkers located between the fluorescent and sensory components. (c) Schematic representation of the NTnC indicator formation and functioning. The mNeonGreen component is shown as a cylinder, which reversibly quenches upon binding with two Ca2+ ions (red dots).

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