Figure 4: Fluorescence changes in response to intracellularly induced APs in cultured neurons expressing the indicators NTnC and GCaMP6s. | Scientific Reports

Figure 4: Fluorescence changes in response to intracellularly induced APs in cultured neurons expressing the indicators NTnC and GCaMP6s.

From: A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

Figure 4

(a) Response to a single AP. (b) Response to a train of 10 APs at 50 Hz. All responses were averaged across all recorded neurons in different wells. Note similar signal amplitudes and response kinetics induced by single APs in NTnC and GCaMP6s cells. (c,d) Dependence of the amplitudes of responses induced by different numbers of APs in neurons expressing NTnC (N = 6) and GCaMP6s (N = 7). Note that in the range from 1 to 10 APs, dependence is linear for both sensors. The linear regression shown in the figure was calculated for the 1–10 APs subset for NTnC and for the whole data range (1–20 APs) for GCaMP6s. Values are shown as the means ± SEM.

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