Table 2 Characteristics of calcium ion responses to intracellular stimulation with 1 and 10 APs in neurons expressing the sensors NTnC and GCaMP6s in dissociated neuronal culture.

From: A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

Protein

Number of cells

APs

Rise half-time, s a

Decay half-time, s b

ΔF/F0c

SNR d

NTnC

6

1

0.15 ± 0.02

2.7 ± 0.5

0.027 ± 0.008

18 ± 4

8

10

0.21 ± 0.01

4.5 ± 0.8

0.15 ± 0.02

80 ± 20

GCaMP6s

5

1

0.19 ± 0.03

2.7 ± 0.3

0.022 ± 0.008

25 ± 9

5

10

0.26 ± 0.01

3.6 ± 0.5

0.60 ± 0.20

400 ± 200

  1. aRise half-time was measured as the time between the stimulus onset and the half-peak of response.
  2. bDecay half-time was calculated as the time from the peak to the half-peak at the end of the response.
  3. cΔF/F0 was calculated as (F − F0)/F0, where F0 is the baseline fluorescence signal averaged over a 1-s period immediately after the start of imaging.
  4. dSignal-to-noise ratio (SNR) was quantified as the peak ΔF/F0 response over the standard deviation of the signal during a one-second period before stimulation. Values are shown as the means ± standard errors of the mean.
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