Table 1 FRET efficiency (E%) and donor-acceptor distances r.

From: Inter-isoform Hetero-dimerization of Human UDP-Glucuronosyltransferases (UGTs) 1A1, 1A9 and 2B7 and Impacts on Glucuronidation Activity

 

FRET efficiency E %

Donor-acceptor distance r (nm)

n

CFP/YFP

−1.56 ± 4.8

>10

5

CFP-linker-YFP

17.4 ± 4.8

6.92

21

1A1*1CFP/1A9*1YFP

18.7 ± 8.2

6.74

14

1A1*1CFP/1A9*2YFP

12.3 ± 4.1

7.32

15

1A1*1CFP/1A9*3YFP

24.3 ± 8.3

6.38

9

1A1*1CFP/1A9*5YFP

10.3 ± 7.4

7.57

18

1A1*1CFP/2B7*1YFP

20.7 ± 6.9

6.60

15

1A1*1CFP/2B7*71SYFP

9.94 ± 4.9

7.62

13

1A1 *1CFP/2B7*2YFP

6.41 ± 2.4

8.25

10

1A1 *1CFP/2B7*5YFP

9.38 ± 7.7

7.70

23

1A1*1bCFP/1A9*1YFP

9.29 ± 5.5

7.71

25

1A1*1bCFP/1A9*2YFP

5.33 ± 3.7

8.52

19

1A1*1bCFP/1A9*3YFP

6.51 ± 3.6

8.23

23

1A1*1bCFP/1A9*5YFP

8.10 ± 3.9

7.91

10

1A1*1bCFP/2B7*1YFP

8.00 ± 4.7

7.93

17

1A1*1bCFP/2B7 *71SYFP

3.19 ± 4.4

9.32

20

1A1*1bCFP/2B7*2YFP

5.36 ± 3.6

8.51

11

1A1*1bCFP/2B7*5YFP

−0.74 ± 2.9

>10

10

1A9*1CFP/2B7 *1YFP

17.9 ± 7.3

6.80

16

1A9*1CFP/2B7*71SYFP

11.8 ± 7.5

7.38

12

1A9*1CFP/2B7*2YFP

6.57 ± 2.6

8.21

12

1A9*1CFP/2B7*5YFP

9.53 ± 5.7

7.68

11

1A9*2CFP/2B7*1YFP

10.3 ± 3.7

7.57

8

1A9*2CFP/2B7*71SYFP

9.23 ± 5.5

7.72

25

1A9*2 CFP/2B7*2YFP

12.3 ± 2.1

7.32

13

1A9*2CFP/2B7*5YFP

3.81 ± 4.1

9.04

15

1A9*3CFP/2B7*1YFP

9.03 ± 5.8

7.75

14

1A9*3CFP/2B7*71SYFP

9.31 ± 5.4

7.71

10

1A9*3CFP/2B7*2YFP

4.76 ± 2.9

8.69

10

1A9*3CFP/2B7*5YFP

12.2 ± 4.3

7.33

16

1A9*5CFP/2B7*1YFP

11.4 ± 6.8

7.42

13

1A9*5CFP/2B7*71SYFP

7.71 ± 4.4

7.98

26

1A9*5CFP/2B7*2YFP

4.88 ± 2.3

8.66

17

1A9*5CFP/2B7*5YFP

13.4 ± 7.2

7.20

17

  1. n is the sample size.
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