Figure 2

Immunoblot analysis of thylakoid membranes proteins extracted from leaves.

Line 1, the leaves were exposed to darkness at 25 °C for 2 h; line 2, the leaves were exposed to approximately 6 μmol m⁻² s⁻¹ UV-B at 25 °C for 2 h; line 3, the leaves were exposed to darkness at 25 °C for 2 h and the leaves were then transferred to chilling-light conditions (6 °C, 200 μmol m⁻² s⁻¹) for 6 h; line 4, the leaves were exposed to approximately 6 μmol m⁻² s⁻¹ UV-B at 25 °C for 2 h and the leaves were then transferred to chilling-light conditions (6 °C, 200 μmol m⁻² s⁻¹) for 6 h; line 5, before treatment; line 6, the leaves were exposed to chilling-light conditions for 6 h; line 7, the leaves were exposed to chilling-light conditions in the presence of approximately 2 μmol m⁻² s⁻¹ UV-B for 6 h; Thylakoid membranes (10 μg of chlorophyll) were separated by SDS-PAGE, electroblotted and probed using specific antibodies against PsbO, PsbA and PsaA.