Figure 7

Relative expression level of genes involved in cardenolide biosynthesis after wound treatment.
(A) Effect of wound on expression of genes involved in terpenoid backbone biosynthesis by qRT-PCR. (B) Effect of wound on expression of genes involved in steroid backbone biosynthesis by qRT-PCR. (C) Effect of wound on expression of genes involved in cardenolide biosynthesis by qRT-PCR. Actin was used as internal reference gene and relative abundance of each gene was compared after 3, 6 and 12 hr of wounding. The data represents three independent biological and experimental repeats with standard deviations. *(P < 0.05) and **(P < 0.001) indicate significant difference in gene expression (control vs wound). Full names of genes are mentioned in Supplementary Table 4.