Table 2 Identification of cardiac glycosides by their characteristic fragment ions.

From: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera

S. No.

RT

M.W.

Identified

m/z (Characteristic fragment ions of genin)

Genin unit

Sugar unit

01

5.34

404

Calotropagenin

387,369,351,341,333,323,305

A

—

02

6.78

536

Frugoside

391,373,355,339,337,325,293

C

146

03

6.89

390

Coroglaucigenin

373,355,339,337,325,293

C

—

04

7.85

532

Calotropin

387,369,359,351,341,333,323

A

146

05

9.04

374

Uzarigenin

357,339,321,293

B

—

06

9.46

574

Asclepin

387,369,351,341,333,323

A

188

07

9.47

530

Uscharidin

387,369,351,333,323

A

144

08

9.90

586

Uscharin

323,333,341,351

A

201

  1. A = Calotropagenin, B = Uzaigenin, C = Coroglaucigenin [Structure characterization of all above cardiac glycosides were based on their mass spectrometry based evidence (molecular weights, accurate mass measurement/chemical formula, characteristic MS/MS fragmentation of genin unit) and literature support].
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