Figure 5: Dissociation of epiblast type stem cells by different methods.
From: Efficient long-term cryopreservation of pluripotent stem cells at −80 °C
(A), morphology of epiblast type ID6 porcine iPSC during culture; (B), colonies manually cut by the cell passaging tool to yield uniform sized clumps; (C), colonies dispersed with Gentle Cell Dissociation Reagent for 6 min typically provided clumps of 6–8 cells; (D), colonies dispersed with TrypLE largely yielded single cells. Scale bar = 500 μm.
