Figure 1 | Scientific Reports

Figure 1

From: A 3′-end structure in RNA2 of a crinivirus is essential for viral RNA synthesis and contributes to replication-associated translation activity

Figure 1

LCV genome organization and layout of the 3′ non-coding region.

(a) A schematic representation of the LCV genome. Open reading frames (ORFs; 1a – 3 and 1–10 in LCV RNAs 1 and 2, respectively) encoding the following predicted viral proteins are as indicated: P-Pro, papain-like protease; MTR, methyltransferase; HEL, RNA helicase; RdRp, RNA-dependent RNA polymerase; HSP70h, heat shock protein 70 homolog; CP, major coat protein; CPm, minor coat protein; and proteins that are named according to their relative molecular masses (indicated by numbers preceded by “P”): P8, P23, P5.6, P6, P6.4, P60, P9, P27 and P4.8. Black bars below the genome map represent DIG-labeled riboprobes (II and VIII) complementary to the corresponding locations in the genomic RNAs. (b) Enlargement of the areas indicated by the dashed-circles (in Fig. 1a) representing the 3′ terminal region of LCV RNAs 1 (top) and 2 (bottom). Numbers above the arrows indicate the nucleotide positions on both RNAs.

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