Figure 3
Inhibition of transport of cysteine mutants by CuPh.
(A) Dose-response effect of CuPh on D-[3H]-Asp transport activity of CL-GLT-1, I93C/V241C and I97C/V241C. (B) Inhibition of transport by 300 μM CuPh is also shown for I93C/V241C and I97C/V241C double mutants, as well as for transporter proteins harboring corresponding individual mutants. The bars marked “I93C co V241C” and “I97C co V241C” represent individual mutants that were co-transfected. In each case, cells expressing mutants and CL-GLT-1 were treated with CuPh in NaCl solution for 5 min before the transport assay. Data represent the percentages of the remaining uptake activity of samples relative to those without any CuPh treatment (mean ± S.E., n = 3). Values that are significantly different from those of CL-GLT-1 were determined by one-way ANOVA (*P < 0.05; **P < 0.01).
