Figure 4
The restorative effect of DTT on the CuPh-mediated inhibition of double mutants.
HeLa cells expressing I93C/V241C (A) or I97C/V241C (B) were preincubated for 5 min in the presence and absence of 20 mM DTT and then incubated with or without 300 μM CuPh and D-[3H]-Asp uptake was measured. Values are shown as a percentage of the uptake of mutants without CuPh and DTT treatment and represent means ± S.E for at least three independent experiments. Values that are significantly different from that of the control were determined by one-way ANOVA (*P < 0.05; **P < 0.01).
