Figure 6
Effect of external media on the CuPh-mediated inhibition of the transport activity of double mutants.
HeLa cells expressing I93C/V241C (A) or I97C/V241C (B) were preincubated for 5 min in external media containing one of the following: NaCl, NaCl + 1 mM L-glutamate, KCl, choline chloride, lithium chloride or NaCl + 20 μM TBOA. The cells were treated in the absence or presence of 50 μM CuPh and then, D-[3H]-Asp uptake was measured. Values are shown as a percentage of the uptake of double cysteine mutants without CuPh treatment and represent means ± S.E for at least three independent experiments. Values that are significantly different from that of the control were determined by one-way ANOVA (*P < 0.05; **P < 0.01).
