Figure 3 | Scientific Reports

Figure 3

From: DNA annealing by Redβ is insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions

Figure 3

Point mutations of Redβ C-terminus show reduced recombination efficiencies.

(a) Western blot analysis showing equivalent protein expression levels of the seven Redβ point mutants. The upper panel shows the 12% SDS-PAGE stained with Coomassie Brilliant Blue above the Western probed with an anti-Redβ antibody, which is quantified below (wt Redβ = 100%). (b) The indicated Redβ point mutations, expressed from pSC101-BAD-Redβ plasmids, were evaluated using the ssOR assay described in Fig. 1c,d. (c) The indicated Redβ point mutations, expressed from pSC101-BAD-Redγβα plasmids, were evaluated using the Beta recombination assay described in Fig. 1e,f.

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