Table 1 Mass distributions for wt, C3 and N1Redβ after size exclusion chromatography.

From: DNA annealing by Redβ is insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions

Protein

Species 1

Species 2

Species 3

Rh (nm)

Dist (%)

~Mw (kDa)

No. of Subunits

Rh (nm)

Dist (%)

~Mw (kDa)

No. of Subunits

Rh (nm)

Dist (%)

~Mw (kDa)

No. of Subunits

WtRedβ

13.5 ± 4.4

31.8

490

11–12

—

—

—

—

4.5 ± 0.6

68.2

29 ± 4

1

C3Redβ

15.7 ± 3.6

45.1

520

12–13

8.3 ± 1.5

45.6

58

2–3

4.0 ± 1.4

9.3

26 ± 1

1

N1Redβ

15.1 ± 0.6

84.3

512

12–13

9.3 ± 0.6

8.3

56

2–3

3.9 ± 0.8

7.4

22 ± 3

1

  1. The wt Redβ, C3Redβ and N1Redβ peaks fractionated by size exclusion chromatography (Fig. 2c), were analyzed by DLS and SLS, which revealed two (wt) and three (C1, N1) species as summarized with hydrodynamic radius (Rh) and distributions (Dist) as percentages together with estimated molecular weights and the corresponding number of Redβ molecules per species.
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