Figure 3
Identification of a HS-site in the C-terminal end of perlecan.
Extracted-ion current chromatograms of a heparinase-digested perlecan sample show residual HS-saccharide glycopeptides originating from to the C-terminal perlecan site. The HS-structures shown are hexasaccharides (m/z 1095.41; 3+), octasaccharides (m/z 1221.78; 3+) and decasaccharides (m/z 1348.48; 3+). Notably, the small peaks eluting at ~46 min relates to NH3-adducts variants of the native structures.
