Figure 1: An example of a digital PCR histogram and scatterplot generated using QuantStudio 3D AnalysisSuite Cloud Software. | Scientific Reports

Figure 1: An example of a digital PCR histogram and scatterplot generated using QuantStudio 3D AnalysisSuite Cloud Software.

From: Comparison of standard, quantitative and digital PCR in the detection of enterotoxigenic Bacteroides fragilis

Figure 1

(a) Representative histogram of bft gene detected using dPCR from a clinical stool sample (in blue); the yellow peak represents wells on the dPCR chip containing no target. The histogram is generated by plotting fluorescence versus well count for every well on the dPCR chip. The red vertical line represents the call threshold calculated by the software. This can be manually user defined to give an acceptable balance of quality and quantity data. (b) The corresponding scatter plot, showing each of the wells with target amplification (blue) or no target (yellow) as a discreet dot. There is acceptable definition between amplified and negative wells; this corresponds to the definition between histogram peaks.

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